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  • 无菌室工作规程应该注意什么?
    来源:http://www.csvchb.com 浏览: 发布日期:2020-06-17
    (1)无菌室灭菌。每次使用前开启紫外线灯照射30min以上,或在使用前30min,对内外室用5%石炭酸喷雾。
    (1) Sterile room sterilization. Before each use, turn on the ultraviolet lamp for 30min or 30min before use, and use 5% carbolic acid spray in the interior and exterior chambers.
    (2)用肥皂洗手后,把所需器材搬入外室;在外室换上已灭菌的工作服、工作帽和工作鞋,戴好口罩,然后用2%煤酚皂液将手浸洗2分钟。
    (2) After washing hands with soap, move the required equipment into the outer room; put on sterilized work clothes, work caps and work shoes in the outer room, wear masks, and then wash hands with 2% coal phenol soap for 2 minutes.
    (3)将各种需用物品搬进内室清点、就位,用5%石炭酸在工作台面上方和操作员站位空间喷雾,返回外室,5~10min后再进内室工作。
    (3) move all necessary articles into the inner room for inventory and position. Spray the 5% carbolic acid on the working table and operator's space, return to the outer room, and then enter the inner room after 5 ~ 10min.
    (4)接种操作前,用70%酒精棉球擦手;进行无菌操作时,动作要轻缓,尽量减少空气波动和地面扬尘。
    无菌室装修
    (4) Before inoculation operation, wipe hands with 70% alcohol cotton ball; during aseptic operation, move gently to minimize air fluctuation and ground dust.
    (5)工作中应注意安全。如遇棉塞着火,用手紧握或用湿布包裹熄灭,切勿用嘴吹,以免扩大燃烧;如遇有菌培养物洒落或打碎有菌容器时,应用浸润5%石炭酸的抹布包裹后,并用浸润5%石炭酸的抹布擦拭台面或地面,用酒精棉球擦手后再继续操作。
    (5) Pay attention to safety in work. If the cotton plug catches fire, hold it tightly or wrap it with a wet cloth to extinguish it. Do not blow it with your mouth, so as not to expand the combustion. If the bacteria culture is spilled or smashed into the bacteria container, wrap it with a rag soaked with 5% carbolic acid, wipe the table or ground with a rag soaked with 5% carbolic acid, and then continue the operation after wiping your hands with an alcohol cotton ball.
    (6)工作结束,立即将台面收拾干净,将不应在无菌室存放的物品和废弃物全部拿出无菌室后,对无菌室用5%石炭酸喷雾,或开紫外线灯照射30min。
    (6) at the end of the work, clean up the table immediately, and remove all the items and wastes that should not be stored in the asepsis room, then irradiate 30min with 5% carbolic acid spray or ultraviolet lamp.
    本文由无菌室装修友情奉献.更多有关的知识请点击:http://www.csvchb.com我们将用真诚的态度.为您提供为全面的服务.更多有关的知识我们将会陆续向大家奉献.敬请期待.
    This article is dedicated by aseptic room decoration. For more information, please click: http://www.csvchb.com We will use our sincere attitude to provide you with comprehensive services. We will continue to offer you more relevant knowledge. Please look forward to it